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KMID : 0043320080310101265
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Archives of Pharmacal Research
2008 Volume.31 No. 10 p.1265 ~ p.1273
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The Retention Behavior of Ginsenosides in HPLC and Its Application to Quality Assessment of Radix Ginseng
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Jiang Zhi-Hong
Wang Wan
Zhao Zhong-Zhen
Luo Guo-An
Hu Ping
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Abstract
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This study systematically investigated the retention behavior of seven neutral ginsenosides Rg1, Re, Rf, Rb1, Rb2, Rc, Rd, and an acidic ginsenoside R0, the major pharmacologically active components of Radix Ginseng with RP-HPLC. The effects of solvent, pH value, ionic strength of the mobile phase, and column temperature were investigated using an octadecylsiloxane-bonded silica gel column. Based on the ginsenosides’ retention characteristics, the concentration of acetonitrile and the gradient of the mobile phase needed to maintain the baseline separation of the major neutral ginsenosides in Radix Ginseng were theoretically predicted. Furthermore, the ionic strength of mobile-phase necessary to achieve good resolution of the neutral ginsenosides and acidic ginsenosides was carefully investigated. According to the results of the quantitative analysis of ginsenosides in eight batches of ginseng samples from different sources, the developed HPLC technique may be a valuable tool for the quality assessment of Radix Ginseng.
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KEYWORD
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Ginsenoside, HPLC, Retention behavior, Radix Ginseng, Quantitation, Panax
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